Synthesis of Urchin-Shaped Gold Nanoparticles Utilizing Green Reducing and Capping Agents at Different Preparation Conditions: An In Vitro Study

Employing environmentally friendly reducing and capping materials to synthesize gold nanoparticles is an exciting research point. However, the used usually need a long reduction time that can take days. In this work, instantaneous production of small-sized (less than 20 nm) investigated using ascorbic acid, gelatin, mixture two agents at different preparation conditions (at room temperature; ± 3 °C near boiling 95 °C). Particle size analysis, as well transmission electron microscopy, were assess produced particles’ physical characteristics. The structural changes optical characteristics monitored UV–visible spectroscopy. Fourier Transform Infrared spectroscopy (FTIR) was establish presence gelatin coating over nanoparticles. morphology spherical with ranging from 8–18 nm, whereas urchin-shaped 24–100 nm formed reaction temperature. hydroxyl amine groups associated confirmed FTIR. This could be step for wider usage green synthesized nanogold particles in several applications.